Name: ARTHUR FAGUNDES DOS SANTOS TEODORO
Publication date: 27/02/2018
Advisor:
Name |
Role |
|---|---|
| SÉRVIO TÚLIO ALVES CASSINI | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| SÉRVIO TÚLIO ALVES CASSINI | Advisor * |
Summary: Lipases are enzymes responsible for the catalysis of reactions from a wide variety
of industrial processes. Solid fermentation appears as an alternative for the
production of enzymes due to the low cost and possibility of using residues of low
value and with great abundance, besides requiring a smaller amount of energy in
comparison to the submerged fermentation. This work had as objective to produce
lipases by the fungi Penicillim sp. and Rhizomucor sp. by solid fermentation using
sugar cane bagasse and coconut fiber. Initially, preliminary tests were carried out
to test the use of a nutrient solution. After the tests, the enzyme production was
optimized through an experimental design 33, WHERE the influence of the inducer
concentration (soybean oil) was studied in 2.5%, 5% and 10%, at a temperature of
27 ° C, 30 ° C and 33 ° C, and 40%, 60% and 80% field retention capacity for each
medium and fungus. With the use of sugarcane bagasse as a support medium,
Penicillium sp presented the highest lipase activity (147,5 U/g) at 30°C
temperature, 60% field capacity and 5% of the inductor, with the Inductor
Concentration and Temperature with significant influence on the results.
Rhizomucor sp had the highest lipase activity (161,3 U/g) under the same
conditions mentioned above and with the same significant influence on the results.
For coconut fiber, Penicillium sp presented the highest lipase activity (75 U/g)
under conditions of 30 ° C temperature, 60% humidity and 10% of the inductor,
with field capacity, concentration of the inductor and temperature with significant
influence on results. Rhizomucor sp had the highest lipase activity (78,9 U/g)
under the same conditions mentioned above and with the same influence in the
results.
